高效液相色谱法测定5-氨基水杨酸结肠溶胶囊的含量

HPLC determination of 5-ASA in colon-release capsules

目的 :测定 5 氨基水杨酸结肠溶胶囊的含量。方法 :采用HPLC法 ,填充剂 :ODSC18(2 5 0mm× 4 .5mm ,5 μm) ;流动相 :A 乙腈 (77∶2 3,其中A为四丁基硫酸氢铵 醋酸钠 =3.4 g∶1.4 g,配成 10 0 0ml) ;检测波长 :2 4 0nm ;流速 :1ml·min-1,进样量 :2 0 μl。结果 :5 ASA平均回收率为 10 0 .18,RSD为 0 .75 %。结论

OBJECTIVE To determinae of 5 ASA in colon release capsules.METHODS High performance liquid chromatographic was used. RESULTS A mean recovery of 100.1% was obtained. CONCLUSIONS An accurate method for determination of 5 ASA in colon release capsules is established.