NFκB家族蛋白对人卵巢癌细胞系生长及耐药性的影响

Effect of NFκB family proteins on growth and drug-resistance of human ovarian cancer cell lines

目的 :研究NFκB家族蛋白在卵巢癌细胞的表达及与卵巢癌细胞化疗耐药性关系。方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。结果 :①P6 5、P5 0及IKB在胞质内表达阳性率分别为 76 .9%、81.8%、84 .6 % ,P6 5、P5 0同时在胞核内表达 ,P5 0尤为明显 ,阳性率分别为 15 .3%、4 5 .5 %。②OV MZ 5和OV MZ 2 774两个卵巢癌细胞系P6 5表达阳性 ,MTT检测IC50 表现明显高值 ,显示耐药。结论 :P6 5、P5 0在卵巢癌细胞的发生、发展中发挥作用 ,P5 0更为明显 ;P6 5与卵巢癌细胞的耐药关系密切 。

Purpose:To investigate the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell lines. Methods:First, 13 ovarian cancer cell lines were cultured, and then protein was extracted separately from cytoplasm and nucleus. Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.Results:It was found that the expression of positive rate of P65, P50 and IKB in cytoplasm was 76.9%, 81.8% and 84.6% respectively, and the significant positive expression of P65 and P50 was also found in the nucleus, with a rate of 15.3%, 45.5% respectively. In OV MZ 5 and OV MZ 2774, the expression of P65 was positive and their IC 50 reached a significant value. Conclusions:It was concluded that P50 and P65 affected the growth and development of ovarian cancer cells with the effect of P50 being more obvious. P65 had a close relationship with the chemotherapy resistance of ovarian cancer cells, and thus P65 could be expected to be a new marker in the observation of prognosis.