牛磺酸对家兔肾上腹主动脉阻断所致急性肾缺血/再灌注损伤保护作用的实验研究

Protective effects of taurine on acute renal ischemia-reperfusion injury during suprarenal abdominal aortic crossclamping in rabbits

目的 探讨牛磺酸对家兔肾上腹主动脉阻断所致急性肾缺血 /再灌注损伤 (ARIRI)的保护作用及可能机制。方法 将 2 4只家兔随机等分为 3组 (每组n =8) :假手术组 (Ⅰ组 )、缺血再灌注组 (Ⅱ组 )和牛磺酸治疗组 (Ⅲ组 )。Ⅱ、Ⅲ两组建立肾上腹主动脉阻断所致ARIRI的动物模型 ,Ⅰ组不阻断主动脉血流。Ⅲ组于阻断主动脉前 5min静注牛磺酸 15 0mg/kg。Ⅰ、Ⅱ组以等容积生理盐水取代牛磺酸。动态检测血中BUN、Cr、SOD、MDA、AT -Ⅱ、尿 β2 -MG的变化 ,以及肾皮质中SOD、MDA的浓度。结果 Ⅲ组在灌注期血BUN、Cr、AT -Ⅱ及尿中β2 -MG含量明显低于Ⅱ组 (P <0 0 5或P <0 0 1) ,血和肾皮质MDA浓度明显低于Ⅱ组 (P <0 0 1) ,SOD活性显著高于Ⅱ组 (P <0 0 1)。Ⅱ组电镜下见明显肾小管损伤及坏死 ,而Ⅲ组肾小管损伤轻微。结论 牛磺酸对家兔肾上腹主动脉所致ARIRI具有良好的保护作用 ,作用机制与其抗氧化作用及抑制AT

Objective To evaluate the protective effect of taurine on acute renal ischemia-reperfusion injury during suprarenal abdominal aortic crossclamping in rabbits and study its probable mechanism Methods 24 rabbits were randomly divided into false operating group(Ⅰ,n=8),ischemia-reperfusion group(Ⅱ,n=8),and Taurine group(Ⅲ,n=8) Model of acute renal ischemia-reperfusion injury (ARIRI) induced with suprarenal abdominal aortic crossclamping was founded in group Ⅱ and group Ⅲ.The abdominal aorta was not occlused in group I. Taurine 150 mg/kg was given intravenously 5 min prior to clamping in group Ⅲ The rabbits in group Ⅰ and group Ⅱ received normal saline as control Such parameters as serum BUN?Cr?SOD?MDA?AT-Ⅱ and urine β 2-MG were measured continually,as well as SOD?MDA concentration in renal cortex Results Comparing with group Ⅱ,in group Ⅲ serum BUN?Cr?AT-Ⅱ and urine β 2-MG concentration after reperfusion were significantly decreased(P<0 05 or P<0 01),Serum and renal cortex MDA concentration were significantly decreased(P<0 01),SOD activity increased obviously (P<0 01) Under electron microscopy,renal pathology associated with acute tubular injury and necrosis were observed in group Ⅱ Meanwhile It was obviously alleviated in group Ⅲ Conclusions Taurine can effectively protect rabbit kidneys against ischemia reperfusion injury during suprarenal abdominal aortic crossclamping.Its mechanism may relate with that Taurine have antioxygenize capability and can inhibit AT-Ⅱ production