缺血预处理中释放的氧自由基对肝脏缺血再灌注损伤保护作用的实验研究

PROTECTIVE EFFECTS OF OXYGEN RADICALS RELEASED DURING ISCHEMIC PRECONDITIONING ON ISCHEMIA/REPERFUSION INJURY OF RAT LIVER

目的 :检验预处理对肝脏缺血再灌注损伤的保护作用 ,研究氧自由基对缺血预处理的作用。方法 :采用大鼠肝脏原位缺血再灌注模型 ,评价缺血预处理中释放的氧自由基对大鼠肝脏缺血再灌注损伤的保护作用。结果 :缺血预处理明显减少AIJ、AST及LDH漏出 ,减少ATP消耗。氧自由基清除剂MPG可以完全破坏缺血预处理的保护作用。氧自由基可以模拟缺血预处理的保护作用 ,而蛋白激酶C抑制剂多粘菌素B可以阻断氧自由基的保护作用。结论 :缺血预处理中释放的氧自由基可以诱导缺血预处理的保护作用 ,蛋白激酶C参与了该保护机制。

Objective:To determine the protective effects of oxygen radicals released during ischemic preconditioning (PC) on ischemia/reperfusion injury.Methods:Using the model of liver subjected to the in situ ischemia reperfusion, the protective effects of oxygen radicals released during ischemic preconditioning(PC) on ischemia/reperfusion injury were investigated.Results: The results showed that PC reduced leakage of ALT, AST and LDH and consumption of ATP. MPG abolished protection of PC. Infusion of Hypoxanthine and xanthine oxidase can mimic protection of PC,and protection elicited by the oxygen radical generating system was abolished by the PKC inhibitor polymycin B. Conclusion:It is suggested that oxygen radicals released during PC can induce protective effects of PC,and PKC might be involved in the protective mechanism.