大鼠肝缺血再灌注后不同时相ICAM-1分子表达和肝损伤的关系

RELATION BETWEEN EXPRESSION OF ICAM-1 AND DEGREE OF LIVER INJURY AFTER LIVER REPERFUSION AT DIFFERENT TIME IN RATS

目的 :探讨大鼠肝缺血再灌注后不同时相细胞间粘附分子 (ICAM - 1)表达和肝损伤的关系。方法 :Wis tar大鼠随机分为假手术组、再灌注 30min组、再灌注 6 0min组、再灌注 2 4h组、再灌注 72h组共 5组 ;建立大鼠肝缺血再灌注模型 ,观察不同时相血清丙氨酸转氨酶 (ALT)和天冬氨酸转氨酶 (AST)水平 ,以及应用免疫组化方法 (SP法 )检测肝组织中不同时相ICAM - 1分子的表达。结果 :大鼠血清ALT和AST水平在再灌注30min升高显著 (P <0 .0 5 ) ,再灌注 6 0min组达高峰 (P <0 .0 1) ,再灌注 2 4h、72h组血清ALT和AST水平降至正常水平 ;再灌注后不同时相ICAM - 1分子表达水平与血清ALT和AST水平呈显著正相关 (P <0 .0 5 )。结论 :ICAM - 1分子参与肝再灌注损伤的过程 ,其表达的强弱与肝损伤程度密切相关。

Objective:To investigate the relationship between the expression of ICAM-1 and the degree of liver injury after liver reperfusion at different time in rats.Methods:Wistar rats were randomly devided into 5 groups:sham-operated group;reperfusion 30min group; reperfusion 60min group; reperfusion 24h group;reperfusion 72h group.The model for reperfusion was established. We observed the level of the aminotransferase in serum and the expression of ICAM-1 in liver tissue after liver reperfusion injury at different time in rats.Results:Compared with sham-operated rats,the level of the aminotransferase in serum after reperfusion 30min, 60min was much higher ( P < 0.05).The level of the ALT and AST in serum is positively correlate with the expression of ICAM-1 ( P <0.05).Conclusion:The degree of ICAM-1 expression is positively correlate with the state of liver reperfusion injury. ICAM-1 takes part in the process of liver ischemia-reperfusion injury.