一氧化氮代谢对缺血缺氧脑组织丙二醛的影响

Effects of L-Arginine on Cerebral Malodialdehyde in Brain With Ischemia and Hypoxia

目的 :探讨一氧化氮 (nitricoxide ,NO)代谢对缺血缺氧脑影响的机制。方法 :在鼠缺血缺氧之前应用NO底物左旋精氨酸 (L arginine,L Arg)和NO合酶抑制剂N 硝基 L精氨酸甲酯 (L nitro argininemethylester,L NAME) ,分别应用硝酸还原酶法和硫代巴比妥酸法测定缺血缺氧后及给药后的NO和丙二醛 (malodialdehyde,MDA)的水平。结果 :给L NAME组与缺血缺氧组比较 ,脑组织MDA水平增加 ,脑水肿明显 ;而给L Arg组的结果相反。结论 :NO代谢对缺血缺氧脑有影响作用 ,L Arg对缺血缺氧脑有保护作用。

Objective: This study was to study the effect of metabolism of nitric oxide (NO) on brain with ischemia and hypoxia. Methods: We used NO substract and inhibitor of NO synthetases L-nitro-arginine methylester (L-NAME). Before ischemia and hypoxia occurred in rats' brains, we used nitric acid reductase method and thiobarbituric acid method to measure the levels of NO and malodialdehyde (MDA) after ischemia and hypoxia group as well as after using drugs. Results: Compared with ischemia and hypoxia group, L-NAME group had increased MDA level and obvious edema in brain. However, the results in L-Arg group was the opposite. Conclusion: The metabolism of NO has effect on ischemia and hypoxia. The L-Arg had protective effect on ischemia and hypoxia.