链脲霉素诱导糖尿病大鼠的早期视觉电生理改变

Electrophysiological changes of streptozotocin-induced diabetic rats in early stage

目的 观察糖尿病大鼠病程早期的视网膜电图变化 .方法 按照国际临床视觉电生理学会的标准化方案 ,采用 REPIport系统和银 -氯化银角膜电极以及不锈钢针状电极对链脲霉素诱导的糖尿病大鼠模型进行 2 wk的暗适应视网膜电图 (scot- ERG)、振荡电位 (OPs)、明适应视网膜电图(phot- ERG)和 30 Hz闪烁反应记录 (30 Hz flicker) .结果 大鼠 scot- ERG的 a波和 b波和 OPs的 O2 波的潜伏期 ,在 1wk时 [a波 (2 7.2± 1.9) ms,b波 (72± 6 ) ms,O2 波 (38± 4 )ms],与未打药时相比 [a波 (2 4 .1± 1.2 ) ms,b波 (6 4 .6±2 .9) m s,O2 波 (34.1± 1.6 ) ms],显著延长 ,2 wk时潜伏期继续增加 [a波 (2 8.4± 1.9) ms,b波 (76± 10 ) ms,O2 波 (41± 6 ) ms],幅值一直没有显著改变 .结论 视觉电生理学检查特别是 scot- ERG和

AIM To observe early electophysiological changes in STZ induced diabetic rats. METHODS According to International Society for Clinical Visual Electrophysiology's standard program, we recorded the scotopic electroretino graph (scot ERG), oscillatory potentials (OPs), photopic electroretinograph (phot ERG) and 30 Hz flicker response in diabetic rats for 2 weeks, by using REPIport system, Ag AgCl corneal contact electrode and stainless steel needle electrode. RESULTS At 1 week after the onset of diabetes, the implicit time of dark adapted ERG's a and b waves and oscillatory potentials' O 2 wave in rats was delayed significantly and continued to delay with the duration of the disease. CONCLUSION Electrophysiology, especially the scot ERG and OPs is a reliable method for detecting out incipient diabetic retinopathy.