LIF和IGF-1对牛胚胎干细胞分离的影响

Influences of LIF and IGF-1 on the isolation of bovine ES cells

比较了添加 L IF,IGF- 1和 L IF+IGF对牛胚胎干细胞 (ES)分离的影响。结果表明 :1添加10 ng/m L L IF能提高胚胎贴壁率 ,促进内细胞团 (ICM)和 ES细胞的增殖并抑制其分化 ;2添加 10 ng/m L IGF- 1既能提高胚胎贴壁率 ,又能使 ICM明显增殖的时间和传代后集落出现的时间提前 ;3添加 10 ng/m L L IF+10 ng/m L IGF- 1,可以同时发挥两种生长因子的作用效果 ,更有利于牛胚胎干细胞的分离。

In this paper,we compared the influences of LIF,IGF 1 and LIF+IGF 1 on the isolation of bovine ES cells.The results showed that:① Supplementing with 10 ng/mL LIF could enhance the ration of embryo attachment,promote the proliferation of ICM and ES cells and inhibit the differentiation of ICM and ES cells;② Supplementing with 10 ng/mL IGF 1 could move up the time for the sufficient proliferation of ICM and ES cells.③ Supplementing with 10 ng/mL LIF+10 ng/mL IGF 1,both the effects of LIF and IGF 1 were brought into play.