摘要
本文报道了1种新的鱼类染色体研究方法——限制性内切酶显带技术。我们应用限制性内切酶Bsp631等分别对黄鳝和长春鳊的染色体进行显带处理,结果表明,Bsp631能使这两种鱼的染色体发生稳定的带纹分化:适度的处理产生多重的G-带状带型,而过度的处理则产生特殊的限制性内切酶抗性带型。根据显带结果分析,我们对鱼类染色体限制性内切酶显带的可行性和实用价值进行了讨论。
The chromosomes of Monopterus albus and Parabramis pekinensis were examined for banding patterns after restriction endonuclease Bsp63l treatment and Giemsa staining. It shows that Bsp63I can cause the chromosomes of these fishes to produce specific banding patterns similar to G-bands. The regularity, feasibility and mechanism of banding patterns with restriction en-donucleases in fish chromosomes are discussed.
基金
国家自然科学基金
关键词
鱼
限制性内切酶
染色体带
基因组
Fishes, Restriction endonuclease, Chromosome banding
