我国青年人红细胞膜血型糖蛋白变异的筛查

A Screening Survey of Variant Glycophorins on the Erythrocyte Membranes Among Healthy Chinese Young People

采用免疫印迹技术首次调查我国人群红细胞膜血型糖蛋白(Glycophorins; GP)的变种。受检者114人,年龄17—25岁,男80名,女34名。以对GP分子不同区域具特异性的4种抗血清为探针,调查发现3个类型的10例变种,总检出率达8.8％。其中汉族的检出率为5.7％,黎族为25％,其余民族中未检出变种。4例Ⅰ型先证者红细胞膜GP的免疫印迹图谱与st^a表型相似,其中1例经血清学检查确证。1例Ⅱ型缺失δGP。其余5例Ⅲ型变种尚未见有文献报道。7例GP变种红细胞膜的唾液酸含量明显高于正常人。

A screening survey of variant glycophorins (GP) on the erythrocyte membranes was conducted among 114 healthy Chinese young people who belong to several different ethnic groups by immunoblotting techniques. The majority (70) were of the Han ethnic group; 24 out of 41 Hainanese belonged to the Li ethnic group. Ten subjects showed variant GP profiles, with an incidence of 8.8%. The frequency of variants was significantly higher in the Li ethnic group (25%) than in the Han ethnic group (5.7%); none was seen among the other ethnic minorities. Probing with four antibodies specific to different regions of GP molecules revealed three distinct types of GP among the variant individuals. Type I observed in four individuals was reminiscent of that exhibited by Sta glycophorin Among them, in one subject the Sta blood group phenotype was confirmed by serological typing. The absence of 0 GP was noted in another subject carrying type Ⅱ variant GP. Patterns in the remaining five individuals (type Ⅲ) could not be correlated with known GP profiles of variants of MNSs blood system. This study suggests that there may be a high incidence of GP variants among certain ethnic groups in China. The sialic acid contents of erythrocyte membranes in seven GP propositi were significantly higher than those of the control.