大豆对4个大豆花叶病毒株系的抗性及其连锁遗传研究

A Study of the Inheritance and Linkage of Resistance to Four Strains of Soybean Mosaic Virus in Soybeans

利用7个抗大豆花叶病毒1—4个株系的品种(系)与1个丰产感病品种杂交,配制成10个组合。从其P_1、P_2、F_1、F_2、F_3和BC_1F_16个世代的鉴定结果表明:大豆对S(?)、S_c、S(?)和S_h株系的抗性分别受单个显性基因R(?)、R_c、R(?)和R_h控制。R(?)与R_c、R(?)与R_h间的重组率分别为25.4±7.7％和27.3±5.4％;R(?)与R_h、R_a与R_g、R_c与R_g间的重组率分别为23.5±5.2％、41.1±8.5％和46.5±7.5％。4个基因的排列项序为R_g—R_h—R_a—R_c,它们不属第一和第八连锁群。

Ten susceptible X resistant and resistant X resistant crosses were made among eight varieties, of which seven were previously identified as resistant to one to four SMV strains, in order to investigate the inheritance of resistance to the four strains (Sa, Sc, Ss, and Sh) and the linkage relationship among the resistant genes. Seeds from F2 individual plants were randomly divided and planted in two separated nurseries, in which the seedlings were inoculated with two different strains. The genotype of each F?plant was detected from the reaction of its F3 progenies to the two strains. The results showed that the resistance to each of the four strains was conditioned by a dominant gene which was labelled here with Ra, Rc, Rs, and Rh, respecti-vely. All the four loci appeared to be in a same linkage group with the order of Rs-Rh-Ra-Ro. The recombination value between R, and R. was estimated to be 25.4±7.7%, and those between R, and Rh, R, and Rk, Ra and Rg, and Rc and Rg to be 27.3±5.4%, 23.5 ±5.2%, 41.1±8.5%, and 46.5±7.5%, respectively. The genes resistance to S. in Kwa-nggyo and '7222' were determined at the same locus, so were the resistant genes for Se. In addition, this linkage group seemed to be not related with the first and eighth linkage group.