白血病患者可溶性耐药相关钙结合蛋白基因的表达及其临床意义

Expression and clinical implications of the soluble drug resistance-related calcium-binding protein(sorcin)gene in leukemia patients

目的 探讨白血病患者可溶性耐药相关钙结合蛋白 (Sorcin)基因的表达及其与临床耐药和疗效的关系。方法 应用半定量RT PCR检测 95例急性白血病患者、2 7例非白血病患者和健康人的Sorcin基因表达水平 ,并分析了Sorcin基因表达的临床意义。结果 白血病患者Sorcin基因表达高于对照组 ,差异有显著性 (P <0 .0 0 1) ;急性髓系白血病 (AML)复发难治组高于初诊组和完全缓解 (CR)组 ;临床耐药组Sorcin基因表达显著高于非临床耐药组 (P <0 .0 0 1) ;Sorcin基因阳性表达的CR率为2 0 .0 % ,Sorcin基因阴性表达的CR率为 80 .0 % ,差异有显著性 (P <0 .0 0 1) ;AML各亚型Sorcin基因过度表达存在差异 ,以M5的阳性表达率最高。结论 Sorcin基因过度表达与白血病患者临床耐药密切相关 ,是影响预后的重要因素 。

Objective To study the relationship between the expression of soluble drug resistance related calcium binding protein(sorcin) gene and the clinical multidrug resistance in acute leukemia(AL).Methods A semi quantitative reverse transcriptase polymerase chain reaction(RT PCR)was used to investigate the transcription levels of the human sorcin gene in 95 AL patients and 27 controls. Results Sorcin gene expression was significantly higher in AL patients than in normal contrls(P<0.001), and higher in relapsed/refractory acute myeloid leukemia (AML) patients than in those newly diagnosed or in complete remission. Sorcin gene overexpression was significantly lower in non resistant patients than in resistant ones (P<0.001). CR rates of these two groups were 20 0% and 80.0%, respectively. Sorcin gene expression was higher in AML M 5 patients than M 2?M 3?M 4 patients. Conclusion Sorcin gene overexpression is significantly associated with clinical multidrug resistance and prognosis,it is one of the indicators for predicting prognosis of AL patients.