补阳还五汤和黄芪对沙土鼠脑缺血再灌注后脑组织兴奋性氨基酸的影响

Effects of Buyanghuanwu Decoction and Huangqi (Root of Astragalus membranaceus or Astragalus membranaceus mongholicus) on Cerebral Excitatory Amino Acid in the Gerbils Undergone Cerebral Ischemia and Reperfusion

研究补阳还五汤和黄芪对脑缺血及其再灌注损伤与脑组织兴奋性氨基酸（EAA）的影响。采用沙土鼠双侧颈总动脉夹闭脑缺血模型,于缺血后 15min、再灌注48h取脑组织测定Gin、Asp、GA-BA、Gly。结果显示:缺血15min后,脑组织Glu、Asp含量明显升高（P<0.05,P<0.01）,GABA含量升高（P<0.05）,补阳还五汤和黄茂可降低脑组织Glu含量（P<0.05）。缺血后15min再灌注48h后,脑组织Glu及Asp含量显著升高（P<0.05）,补阳还五汤和黄茂均可使脑组织Glu含量降低（P<0.05）。说明补阳还五汤和黄民抗缺血性脑损伤的作用机理可能与其对抗脑缺血及其再灌注后EAA的升高有关。

The effects of Buyanghuanwu Decoction (BD) and Huangqi (the root of Astragalus membranaceus or Astragalus membranaceus mongholicus) on cerebral excitatory amino acid (EAA) were investigated in gerbils undergone cerebral ischemia and reperfusion. Gerbil cerebral ischemia model was established by the method of ligating common carotid arteries. The levels of cerebral glutamate (Glu), asparate (Asp), γ-aminobutyric acid (GABA) and glycine (Gly) were measured in the model. The results showed that in 15 minutes after cerebral ischemia, the levels of Glu, Asp and GABA markedly increased (P <0.05, P <0.01, P < 0.05), and BD and Huangqi could decrease the level of Glu (P < 0.05); in 48 hours after reperfusion following 15 minutes of cerebral ischemia, the levels of Glu and Asp markedly increased (P < 0.05), and BD and Huangqi also could decrease the level of Glu (P < 0.05). The results suggest that the mechanism against ischemic cerebral injury of BD and Huangqi may be related with their resisting the increase of the level of EAA induced by cerebral ischemia and reperfusion.