高脂血清对体外培养小鼠腹腔巨噬细胞的影响及调肝导浊中药的调节作用

Experimental Study of the Effects of Hyperlipidemic Serum on the Culture of Mouse Celiac Macrophages in vitro and the Regulative Effects of TCM Drugs for Relieving Hepatic Depression and Removing Turbidness

体外培养小鼠腹腔巨噬细胞,分别加高脂血清及载药血清,测定不同培养液中NO、NOS、OX-LDL含量。并制备巨噬细胞,提取RNA,采用RT-PCR观察M-CSF mRNA表达。结果表明,调肝导浊中药可显著增强NOS活性,增加NO含量,抑制OX-LDL生成,并对M-CSF具有调节作用。提示调肝导浊中药具有良好的抗氧化修饰反应作用,从而有效防治AS形成发展。

Mouse celiac macrophages (Mφ) were cultured with hyperlipidemic serum and drug-carried serum respectively, and the levels of nitric oxide (NO), nitric oxide synthase (NOS) and oxidized low density lipoprotein (OX-LDL) were measured in the different culture fluids. RNA was extracted from Mφ, and the mRNA expression of macroph-age colony-stimulating factor (M-CSF) was observed by PT-PCR. The results showed that the TCM drugs for relieving hepatic depression and removing turbidness could enhance NOS activity significantly, increase the level of NO, inhibit the production of OX-LDL, and regulate the level of M-CSF, which suggests that these TCM drugs possess good effects against oxidative modification reaction, hence to prevent and treat arteriosclerosis effectively.