中药FESOL抗单纯疱疹病毒Ⅱ型的实验研究

STUDY ON EFFECT OF CHINESE HERBS FESOL ANTI-HERPES SIMPLEX VIRUS TYPE Ⅱ IN VITRO AND IN VIVO

目的 研究中药FESOL抗HSV -Ⅱ的药效学。方法 用MTT法和小鼠阴道模型分别进行了体内和体外抗HSV -Ⅱ实验。结果 MTT法测得FESOLTC50 为 2 .5mg·ml-1,IC50 为 0 .0 31mg·ml-1,治疗指数为 80 .6 5。与ACV相比 ,抑制HSV -Ⅱ的作用无显著性差异。小鼠阴道模型研究结果 ,FESOL高剂量组 (8.33、1.6 7g·kg·d-1)均明显降低感染鼠的发病率、死亡率 ,延长其平均存活天数 ,延缓病变发展速度 ,减轻病变程度。结论 FESOL具有良好的抗HSV

OBJECTIVE To study the effect of Chinese herbs FESOL against Herpes simplex vitus type Ⅱ(HSV-Ⅱ) . METHODS To study the effect of FESOL in vitro and in Vivo by MTT assay and the mice vagina mode infected by HSV-Ⅱ, with acidic guanosine(ACV) as the control drug. RESULTS The study results in vitro showed that TC 50 was 2.5 mg·ml -1,IC 50 was 0.031 mg·ml -1and TI was 80.65 by MTT assay; The study results in vivo showed that FESOL(8.33?1.67?0.34 g·kg -1·d -1)could effectively treat HSV-Ⅱ infection in mice. CONCLUSION FESOL has good effect on inhibiting HSV-Ⅱ in vitro and in vivo.