葡萄籽多酚在拟衰老模型小鼠中抗氧化功能的研究

Anti-oxidative effects of proanthocyanidins in mice induced by D-galactose

为探讨葡萄籽多酚 (PC)在拟衰老小鼠模型中的抗氧化功能 ,将动物按体重随机分组 ,除对照组外 ,每日通过颈背部皮下注射灭菌D -半乳糖 ,同时经口灌胃给予受试物或对照物 ,阳性对照组给与维生素E ,连续 6 0天后检测结果 :模型对照组相对于空白对照组血清、肝、脑中丙二醛 (MDA)水平上升 ;血、肝脏超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH PX)活性分别下降 ;脑中单胺氧化酶B(MAO B)水平上升 ,肝中MAO B水平无明显变化。经灌胃给予PC剂量分别为 2 5、5 0、10 0mg kgBW的抗氧化效果为使血清、肝脏、脑组织中LPO水平均有不同程度的下降。血、肝中SOD活性水平有不同程度的上升。脑MAO B活性水平下降 ;肝脏MAO B活性水平基本无变化。维生素E组亦有不同程度的抗氧化作用。本次实验结果显示 ,PC具有良好的抗氧化功能 ,能在一定程度上延缓由于注射D

The anti oxidative effect of proanthocyanidins(PC) was evaluated in D galactose induced murine model. Male KUNMING mice were divided into different groups at random. The mice were induced by subcutaneous injection of D galactose on the back of mice daily for 60 days and simultaneously PC and VE were administered to the mice by oral feeding.Results indicate that the lipid peroxide in blood, liver and brain significantly increased respectively because of the D galactose injection, when compared to the control. The activity of SOD in RBC and liver significantly decreased while that of GSH PX in blood and liver decreased. MAO B in brain was significantly increased while MAO B in liver doesn't change significantly. Oral feeding PC markedly decreased the formation of MDA in blood, liver and brain, increased the activities of SOD in blood and liver and decreased the activity of MAO B in brain.Vitamin E also counteract the oxidative stress induced by D galactose. In summary, treatment with PC at all the three tested doses were effective in exerting a protective effect against oxidative stress.