摘要
将经sau3A部分酶切过的菠菜叶绿体DNA(ct DNA),克隆到载体pGA46的BgⅢ位点,得到了具有启动功能的菠菜ct DNA片段,对其中的两个片段进行序列分析后观祭到有与原核生物启动区域相符的保守顺序。由此,本文对片段所具有的启动功能的强弱做了说明。
Sau3A partially digested spinach ctDNA fragments were cloned in BgⅢ restriction site of a E. colt promoter probing vector pGA46. The cloned fragments with promoter activities in E. coli were found by the abilities of recovering tetracycling resistance of their host cells. The promoter functions of the two fragments and their different strengths are deciphered by analyzing their DNA sequences.
关键词
菠菜
叶绿体
脱氧核糖核酸
克隆
Spinach chloroplast DNA, Promoter, Sequencing
