摘要
本文对分布于云南境内的两种Leptolalax——L.ventripunctatus和L.alpinus的常规Giemsa核型、C-带和Ag-NORs作了研究,结果表明L.ventripunctatus的2n=22,20M+2T,NF=42,1对Ag-NORs位于5(?),并呈现异形现象,该区域亦显C-带正染;L.alpinus 2n=24,14M+4SM+6T,NF=42,1对Ag-NORs位于No.8短臂端部,并有随体联合现象。两种的着丝点区域均呈现C-带正染。
The karyotypes, Ag-NORs and C-banding for two Chinese leptolalax pelobatoids (Lep-tolalax ventripunctatus, L. alpinus) collected from Yunnan Province were studied. The results showed that they had 2n = 22(20M + 2T), NF = 42, one pair of secondary constriction and Ag-NORs located on 5q, all centromeric regions of the genome presented C-positive staining, also on the 5q for Leptolalax ventripunctatus. And 2n = 24(14M + 4SM + 6T), NF = 42, one pair of Ag-NORs and secondary constriction located on 8p, on which the satellite association occu-red and all the centromeric regions of their chromosomes were positive in C-banding stain in L. alpinus. It is indicated that the centromeric fussion may take place in the karyotypic change proccess. In addition, the size of heteromorphism of Ag-NORs in L. ventripunctatus has been discovered.No heteromorphic sex chromosomes were discovered in L. alpinus based on the analysis of karyotype and banding techniques.
基金
国家自然科学基金
关键词
掌突蟾
锄足蟾科
细胞遗传学
核型
Leptolalax ventripuctatus, L. alpinns, karyotype, Ag-NORs, C-banding, Pelobatidae
