摘要
对73个昆明白小鼠4-细胞胚胎进行单卵裂球显微活检,活检后84.9%(62/73)的胚胎仍有1/2以上的卵裂球完整;85.5%(53/62)的胚胎经培养发育到胚泡期。取出的40个单卵裂球在含输卵管片段的培养基中培养,并进行体外增殖供制备染色体之用,以此进行细胞遗传学诊断。另对35个活检后胚胎进行超快速冷冻保存,解冻后77.1%(27/35)的胚胎发育至胚泡。证明该模型是可行的。
A technique to kick out single blastomeres from the mouse preimplantation embryos for genetic diagnosis has been developed. A single blastomere was taken from 4-cell mouse embryos by micromanipulative biopsy, 73 embryos were sampled, 62 had half or more blastomeres intact after manipulation (84.9%) and 53 continued to develop into the blastocyst stage in vitro. 35 embryos cryopreserved by ulararapid freezing, after thawing, kept half or more of their blastomeres intact. 27 embryos formed blastocysts in vitro. 40 single blastomeres were co-cultured with a small fragment of mouse oviduct in vitro for blastomere proliferation. 12 of them formed mini blastocysts, and 10 formed 4-8 cell mass. Some of them were karyotyped successfully. These parameters suggest that the technique presented is reilable for preimplantation embryo genetic diagmosis.
出处
《遗传与疾病》
CSCD
北大核心
1991年第3期152-154,192+196,共3页
关键词
产前诊断
遗传学诊断
卵裂球增殖
4-cell mouse embryo Micromanipulative biopsy Cryopreservation Blastomere proliferation Genetic diagnosis
