人胰岛素样生长因子Ⅰ在毕赤氏酵母中的表达研究

Expression of Human Insulin-like Growth Factor I in Methylotrophic Yeast Pichia pastoris GS115

利用PCR定点突变技术对人胰岛素样生长因子（huIGF-I）基因进行改造,克隆到pGEMT载体测序。并构建酵母表达载体pGaAIGF-I,转化毕赤氏酵母GS115。摇瓶发酵4d后,检测表达水平占可溶性总蛋白的15％以上,且表现出较好细胞增殖活性。

The gene encoding human insulin-like growth factor I (huIGF-I) was modified with PCR site-mutation technique and ligated to pGEMT vector and sequenced. Then the yeast expression vector pGaAIGF-I was constructed and transformed to P. pastoris GS115. The expressed huIGF-I reached to 15% of the total soluble yeast proteins and had bioactivity of promoting proliferation of Balb/3T3 cells.