达纳康对新生大鼠缺氧缺血性脑损伤和血小板活化因子含量的影响

Effect of Tanakan on hypoxic-ischemic brain damage and platelet activating factor level in neonatal rat

目的 为达纳康应用于新生儿缺氧缺血性脑病提供更为可靠的理论依据。方法 建立胎鼠宫内缺血模型 ,分别检测假手术对照组、模型组、达纳康治疗组及预处理组鼠仔脑匀浆的PAF含量 ,并观察电镜变化。结果 ①电镜下可看到 :模型组核染色质边集 ,电子密度增加 ,细胞间隙加大。核溶解 ,核膜消失 ,线粒体明显肿胀。并可见到凋亡小体。而在治疗组和预处理组 ,染色质边集程度减轻 ,核膜可见 ,线粒体肿胀明显减轻。并且以预处理组改善明显。而假手术对照组未见染色质边集、线粒体肿胀及核的改变。②各组新生大鼠于生后第 48h检测脑组织PAF含量。模型组PAF含量较假手术对照组明显升高 ,治疗组及预处理组PAF含量较模型组降低 ,且预处理组降低明显 ,均具有统计学意义。结论 达纳康对新生大鼠HIBD具有脑保护作用 。

Objective To investigate the efficacy of Tanakan, Ginkgo biloba extract, in neonatal rat hypoxic ischemic brain damage. Methods Hypoxia ischemia was induced in rat pups by performing a delayed cesarean section on the last day of pregnant Sprague Dawley rats. Tanakan (one kind of Ginkgo biloba extract) 100?mg·kg -1 added to the pups for 2 days. Pregnant at the sixteenth day SD female rats were treated with 100?mg·kg -1 Tanakan for 5 days. Then they were performed the same operation. Litters delivered by cesarean section without producing hypoxic ischemic were used as controls. We examine platelet activating factor content in the pup brain tissues. Results Electron microscopic observations showed hypoxic ischemic changes in the neuron of model rats. And the damage was lessened in the neuron of pretreatment or therapy group. The content of PAF was significant increase in hypoxic ischemic model rats ( P< 0.01 ). Pretreatment or therapy with Tanakan pups showed a significant reduction in PAF levels. And PAF levels in pretreatment pups was lower than that in therapy pups. Conclusion Tanakan can protect the neonatal brain when hypoxia ischemic brain damage happens. And pretreat with Tanakan is better than that of therapy.