实验性重症急性胰腺炎细胞因子的变化规律

Change of cytokines in experimental severe acute pancreatitis

目的 探讨重症急性胰腺炎（severe acute pancreatitis,SAP）大鼠模型细胞因子的变化规律及其与病情严重度的关系。方法 胰管内逆行注射5％牛磺胆酸钠的方法建立SAP大鼠模型于制模后不同时间获取标本,检测模型大鼠血清中IL-2、IL-8、IL-10、TNF-α的浓度变化。淀粉酶测定采用碘比色法,细胞因子的测定采用ELISA法。结果 制模后大鼠腹水量逐渐增多（由0至14.50ml）,胰腺病变评分由0递增至12h的12.83。血和腹水淀粉酶随发病时间而递增。TNF-α、IL-8、IL-10假手术组的浓度很低,制模后上升,第4、5h达到高峰（分别为532.73 mm/ml,360.93pg/ml,193.12 pg/ml）。IL-2假手术组为297.22pg/ml,模型组制模后1h为209.88pg/ml,后逐渐降低,12h为46.16pg/ml。讨论 大鼠SAP发病后,IL-2浓度随胰腺病变的加重逐渐降低,TNF-α、IL-8、IL-10则逐渐上升,在达到高峰后下降。细胞因子的变化影响了SAP病变的严重度,并随病变严重度的变化而变化。

Objective To investigate several pro-inflammary cytokines and change of anti-inflammatory cytokines in the model SAP. Methods SAP animal model was developed in adult male mice by retrograde injection of 5% sodium taurocholate 1 ml/kg in pancreatic duct. Serum amylase was measured by the blue-starch method and serum cytokines were determined by ELISA. Results The ascites volume and amylase increased with delay of time after SAP induction. They had the same tendency as in the case of pancreas injury. TNF-α,IL-8 and IL-10 rose gradually and reached the peak at hour 4 and 5. Differently, IL-2 decreased after SAP. Conclusions In experimental SAP, serum IL-2 is decreased and the other cytokines are increased, reaching the peak at hour 4 and 5.