全身炎性反应综合征患者血清唾液酸水平及免疫功能的变化

Changes of serum sialic acid and T-lymphocyte subsets in patients with SIRS

目的 通过观察全身炎性反应综合征 (SIRS)患者血清唾液酸 (SA)水平及T淋巴细胞亚群含量的变化 ,探讨其临床意义。方法 用酶法测定 60例SIRS患者血清SA水平 ,同时应用荧光单抗CD3+ 、CD4 + 、CD8+ 对血中淋巴细胞进行标记和计数。结果 与对照组相比 ,SIRS患者血清SA、外周血CD8+ 升高 ,其中中、重型组差异有显著性 (P <0 .0 5或 P <0 .0 1 ) ,而CD3+ 、CD4 + 减少 ,其中中、重型组差异有显著性 (P <0 .0 5或 P <0 .0 1 ) ,CD4 + /CD8+ 比值各组均降低 (P <0 .0 5或P <0 .0 1 )。SA与CD4 + 间呈负相关关系 (r =- 0 .387,P <0 .0 5) ,与CD8+ 间呈正相关关系 (r =0 .468,P <0 .0 5)。结论 血清SA水平、外周血T淋巴细胞亚群变化与SIRS病情严重程度有关 。

Objectives To investigate the clinical significance of sialic acid(SA) in serum and T-lymphocyte subsets by observing the change of concentration in blood with systemic inflammatory response syndrome(SIRS).Methods The concentration of SA in serum of 60 patients were measuring with enzyme assay and lymphocyte cell in the blood were marked and counted by mono antibodies CD 3 +?CD 4 +?CD 8 + at the same time.Results The serum SA and CD 8 + in patients with SIRS were increased respectively compared with the controls,especially in middle and severe patients(P<0.05 or P<0.01),while CD 3 +?CD 4 + were decreased respectively in middle and severe patients compared with control groups(P<0.05 or P<0.01).CD 4 +/CD 8 + was decreased respectively contrasted with control groups(P<0.05 or P<0.01).SA had negative correlation with CD 4 +(r=-0.387,P<0.05) while positive with CD 8 +(r=0.468,P<0.05).Conclusions The levels of SA in serum and the change of T-lymphocyte subsets have relation with the degree of seriousness of SIRS.Measuring their concentration would contribute to assess the degree and the prognosis of the patients with SIRS.