血管内皮生长因子在糖尿病大鼠视网膜中的表达

The expression of vascular endothelial growth factor in the retinas of rats with diabetes induced by streptozotocin

目的 :观察血管内皮生长因子 (VEGF)蛋白在链脲佐菌素 (STZ)诱导的糖尿病大鼠视网膜中的表达情况 ,以探讨其在早期糖尿病视网膜病变 (DR)中的作用。方法 :选择健康成年Wistar大鼠 4 0只 ,随机分成正常对照组 (CON)、糖尿病 1个月组 (DM1)、糖尿病 3个月组 (DM3)及糖尿病 6个月 (DM6 )组 ,每组 10只。大鼠腹腔内注射STZ诱发大鼠糖尿病。制备大鼠视网膜石蜡切片行免疫组化ABC法染色 ,光镜观察。结果 :VEGF于CON和DM1组只表达于内核层及节细胞层部分细胞 ;病程 3个月时 ,尚可见视网膜血管的阳性反应 ;6个月时 ,阳性反应范围又扩大至视杆内节和色素上皮细胞。结论 :随病程进展 ,VEGF在视网膜中的表达呈逐渐增强的趋势 ,提示它在早期DR的发生。

Objective:To investigate the expression of vascular endothelial growth factor(VEGF) in the retinas of rats with early diabetes induced by stoptozotocin. The role of retinal cell apoptosis in diabetic retinopathy(DR) was also investigated.Methods:40 healthy adult Wistar rats were chosen and randomly divided into four groups of normal control(CON),diabetes mellitus one month(DM1),three month(DM3) and six month(DM6) groups. Diabetes was induced in the rats by streptozotocin(STZ) administered intraperitoneally. Retinal paraffin sections were stained by immunohistochemical(ABC) method and observed under light microscopy.Results:In CON and DM groups,VEGF's proteins were only expressed in the partial cells of the inner nuclear layer(INL) and ganglion cell layer(GCL). In DM3,VEGF positive reaction was also seen in the retinal vessels. In DM6,VEGF positive reaction was extended further to the inner rod segments and the pigment epithelial cells.Conclusion:The expression of VEGF proteins in the retinas of diabetic rats increases with the progression of diabetes,proving that VEGF plays an important role in the occurrence and development of early diabetic retinopathy.