乙型肝炎病毒感染者人类白细胞抗原的检测

Preliminary report on the influence of HLA classⅡ molecules on outcome of hepatitis B virus infection in Zhejiang district

目的 探讨宿主人类白细胞抗原 (HLA)Ⅱ类分子与HBV感染相关性。方法 利用PCR技术检测 3 0例慢性乙型肝炎患者和 5 6例HBV感染自动恢复者的HLAⅡ类分子及其等位基因。结果 与慢性乙型肝炎患者相比 ,HLAⅡ类分子DR12在恢复者中的分布频率显著增高 (10 %比3 8% ,rr,0 .19;P矫正 <0 .0 2 5 ) ;HLA DR12的等位基因DRB1 12 0 1的分布频率也显著增高 (3 %比3 2 % ;rr,0 .0 7;P矫正 <0 .0 0 5 )。而DR9(4 3 %比 18% ;rr,3 .5 2 ;P矫正 <0 .0 2 5 )和DQ9(4 3 %比 2 0 % ;rr ,3 .13 ;P矫正 <0 .0 5 )在慢性乙型肝炎患者中的分布频率显著高于恢复者。结论 HLA DR12和DRB1 12 0 1可能对机体免受HBV长期感染有保护性意义。而HLA

Objective To investigate correlation between HLA classⅡ molecules and the different outcome of viral hepatitis B. Methods Thirty patients with chronic hepatitis B and 56 subjects who had spontaneously recovered from HBV infection in Zhejiang district were enrolled in this investigation. HLA classⅡ molecule types and their alleles were determined by PCR ssp. Results HLA DR12 was found in 21 of 56 subjects recovered from hepatitis B (38%), compared to 3 of 30 patients with chronic hepatitis B (10%, rr 0.19; P corr <0.025). The frequency of the allele of HLA DR12 DRB1*1201 was found higher in the subjects recovered from hepatitis B (32%) than that in the patients with chronic hepatitis B (3%; rr,0.11; P corr <0.05). On the contrary, HLA DR9 was detected much more in the patients with chronic hepatitis B (43%) than in the subjects recovered from hepatitis B (18%; rr,3.52; P corr <0.025). HLA DQ9 was also detected with a higher frequency in patients with chronic hepatitis B (43%) than in those recovered from hepatitis B (20%; rr, 3.13; P corr <0.05).Conclusions The HLA classⅡ molecule DR12 and its allele DRB1*1201 are associated with protection against chronic hepatitis B in Zhejiang district. HLA DR9 and DQ9 are associated with chronicity of HBV infection.