脑梗死病人血浆凝血因子Ⅶ水平及其基因多态性研究

Study on the Levels of Plasma Coagulation Factor Ⅶ(FⅦ) and Polymorphisms of FⅦ Gene in Patients with Cerebral Infarction.

目的:研究脑梗死病人血浆FⅦ水平及FⅦ基因多态性。方法:测定62例脑梗死病人和149名正常对照者的血浆活化因子Ⅶ（FⅦa）、因子Ⅶ活性（FⅦc）及因子Ⅶ抗原（FⅦ Ag）。应用聚合酶链-限制片段长度多态性（PCR-RFLP）和琼脂糖凝胶电泳法鉴定FⅦ基因中5个可能与血栓有关的多态位点。结果:与正常对照组比较,脑梗死组的FⅦa、FⅦc和FⅦ Ag水平均显著增高,P值分别<0.05、<0.01和w<0.05;两组间各基因型频率和等位基因频率差异均无显著性。结论:血浆中FⅦc水平升高,尤其是FⅦa和FⅦc水平升高可能促进血栓形成而导致发生脑梗死。

Objective: To evaluate the levels of plasma FⅦ and polymorphisms of FⅦ gene in patients with cerebral infarctioa Methods: The plasma level of FⅦa, FⅦ Ag and FⅦc was measured and polymorphisms of FⅦ gene were analyzed in con-trols(149 subjects) and a group of patients with cerebral infarction(62 cases) by combining the methods of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) with agarose gel electrophoresis. Results: The levels of plasma FⅦa, FⅦ Ag and FⅦc in cerebral infarction group were significantly higher than those of the controls and there was no significant difference in genotypic frequencies and allelic frequencies between them. Conclusions: Increasion of the levels of plasma FⅦ,especially FⅦa and FⅦc, may lead to thrombosis and cerebral infarction.