干细胞因子受体在急性白血病病人中的表达及其临床意义

Expression of Stem Cell Factor Receptor in Patients with Acute Leukemia and Its Clinical Significance.

目的:探讨干细胞因子受体（c-kit R）在急性白血病病人中的表达及其临床意义。方法:采用流式细胞仪（FCM）分别检测24例急性淋巴细胞白血病（ALL）和47例急性髓细胞白血病（AML）病人骨髓单个核细胞（MNC）跨膜c-kit R表达的阳性率及阳性水平。结果:ALL病人c-kit R表达的阳性率及阳性水平与正常人相比差异无显著性（P>0.05）,而AML病人的c-kit R水平则显著高于对照组和ALL病人（P<0.01）。AML病人的c-kit R表达率及阳性水平以M1、M2最高,M4、M5最低,两者间差异有显著性（P<0.05）;染色体核型分析显示,c-kit R阳性表达的AML病人,大多数出现染色体核型异常。结论:c-kit R检测有助于ALL和AML的鉴别及AML的FAB亚型分型。

Objective: To explore the expression of human stem cell factor (c-kit R ) in patierts with acute leukemia and to evaluate its clinical significance. Methods; The transmembrane c-kit R expressed on mononuclear cells of bone marrow in 24 patients with acute lymphoid leukemia (ALL) and 47 patients with acute myelogeneous leukemia (AML) was detected by the method of Flow cytometry (FCM). Results: Compared with the normal controls, the expression of c-kit R in group of ALL was not significantly higher(P>0.05), but in group of AML, it was significantly higher than that of the normal controls and group of ALL(P<0.01). Among the FAB subtypes of AML, the expression of c-kit R was significantly higher in M1 and M2 than M4 and M5 (P<0.05). Meanwhile, abnormal karyotype of chromosome occurred in most cases of positive expression of c-kit R. Conclusions: Detecting the expression of c-kit R might be useful to distinguishing ALL from AML and classifying the FAB subtypes of AML.