小剂量阿司匹林与培哚普利合用对兔血中前列环素、血栓素A_2和去甲肾上腺素的影响

Effect of low-dosage aspirin combined with perindopril on prostacyclin, thromboxone A_2, and norepinephrine in rabbits' blood

目的 :检测小剂量阿司匹林 (ASA)与血管紧张素转换酶抑制剂 (ACEI)在单用或合用时兔血中前列环素(PGI2 )、血栓素A2 (TXA2 )和去甲肾上腺素 (NE)浓度的变化 ,探讨它们合用时相互作用的关系。方法 :将 4 0只健康新西兰兔随机分成 4组 ,于连续投药 4周前后分别心脏穿刺取血 ,放射免疫法检测PGI2 和TXA2 ,高效液相色谱法检测NE。结果 :不论是单用还是合用 ,ACEI均使PGI2 浓度增高 (P <0 .0 5 ,P <0 .0 1) ,小剂量ASA则降低TXA2 的浓度 (P<0 .0 5 ,P <0 .0 5 ) ;ASA与ACEI合用组的PGI2 TXA2 明显升高 (P <0 .0 1) ,血清NE浓度明显下降 (P <0 .0 0 1)。结论 :小剂量ASA和ACEI单用时对兔血清NE浓度无明显影响 ,合用时PGI2 TXA2 比值上升 ,血清NE浓度下降 ,故二者有协同作用。

Objective To explore the interaction of low dosage aspirin combined with angiotensin converting enzyme (ACE) inhibitors by prostacyclin (PGI 2), thromboxone A 2 (TXA 2) and norepinephrine (NE)) levels in rabbits' blood. Methods Forty healthy New Zealand rabbits were divided into four groups. Blood samples were drawn from the rabbits' heart before and after a consecutive four week. NE was measured by high performance liquid chromatography, and PGI 2 and TXA 2 were measured by radioimmunoassay. Results ACE inhibitors increased PGI 2 levels (P<0.05, P<0.01); low dosage aspirin suppressed TXA 2 productions (P<0.05, P<0.01) after the four week administration. Aspirin combined with ACE inhibitors led to a significant increase in PGI 2/TXA 2 (P<0.01), together with a significant decrease in NE levels in the rabbits' blood (P<0.001). Conclusion Neither low dosage aspirin nor ACE inhibitors influence NE levels alone. The ratio of PGI 2 to TXA 2 increased, and NE levels decreased significantly during the administration of aspirin combined with ACE inhibitors. The results suggest that there is a synergis action between low dosage aspirin and ACE inhibitors due to increased PGI 2/TXA 2 and decreased NE levels.