TGFβ_1及其受体TGFβ RI在唾液腺腺样囊腺癌组织中的表达

Expression of TGF β_1 and TGF β RI proteins in tissues of salivary adenoid cystic carcinoma

目的 :研究TGFβ1 及其TGFβRI在唾液腺腺样囊腺癌中的作用。方法 :应用免疫组化SP法检测唾液腺腺样囊腺癌中TGFβ1 和TGFβRI的蛋白表达 ,并与正常唾液腺及唾液腺多形性腺瘤作定量比较。结果 :①TGFβ1 和TGFβRI蛋白在正常唾液腺组织中主要表达于各级导管上皮细胞和肌上皮细胞中 ,腺泡细胞无表达 ;②TGFβ1和TGFβRI蛋白在唾液腺多形性腺瘤组织中主要表达于呈腺管状、条索状及团块状排列的肿瘤上皮细胞中 ,其表达水平与正常唾液腺差异无显著性 (P >0 .0 5 ) ;③唾液腺腺样囊腺癌组织中TGFβ1 蛋白表达水平显著增高 (P <0 .0 1) ;TGFβRI蛋白表达水平显著降低 (P <0 .0 1)。结论 :TGFβRI蛋白的低表达在唾液腺腺样囊腺癌的发生发展中起重要作用 ,腺样囊腺癌独特的不良生物学行为可能与TGFβ1 蛋白的过度表达以及TGFβRI蛋白的低表达有关。

Objective To determine the possible role of TGF β 1 and TGF β RI in facilitating neoplastic progression in human salivary adenoid cystic carcinoma (ACC). Methods The expression of TGF β 1 and TGF β RI protein in human salivary adenoid cystic carcinoma was detected by the streptavidin peroxidase (S P) immunohistochemical method, and their contents were compared with those of normal salivary gland tissues and salivary pleomorphic adenoma (PA). Results ①TGF β 1 and TGF β RI proteins were expressed in most ductal epithelial cells and myo epithelial cells, but they were not expressed in the acinar epithelial cells of any normal glands examined; ②TGF β 1 and TGF β RI proteins were expressed in the duct like arranging tumor cells and other solid proliferating tumor cells of salivary pleomorphic adenoma, and no difference of their contents existed between PA and normal salivary gland tissues (P>0.05); ③The contents of TGF β 1 in ACC were significantly higher than those in normal salivary glands (P<0.01), while the contents of TGF β RI in ACC were significantly lower than those in normal salivary glands (P<0.01). Conclusion The abnormal expression of TGF β 1 and TGF β RI might play an important role in tumor genesis and the development of malignant salivary gland tumors such as ACC; the over expression of TGF β 1 and low expression of TGF β RI might involve the bad biological behavior of ACC.