电泳法定量测定血清脂蛋白胆固醇及其临床意义

Electrophoresis in the quantitative determination of cholesterol in lipoprotein fractions and its clinical application

目的 :探讨新电泳法的临床应用价值 ,观察急性冠脉综合征患者脂蛋白谱的特征。方法 :采用脂蛋白胆固醇电泳分析法和目前的常规方法对 2 6例急性冠脉综合征患者和 2 0例稳定型心绞痛患者脂蛋白进行检测。结果 :两种方法测量的脂蛋白胆固醇相关性好 ,且电泳法能直接测定极低密度脂蛋白胆固醇 (VLDL C)。急性冠脉综合症患者VLDL C及甘油三酯较稳定型心绞痛患者明显升高 [(0 4 7± 0 33)mmol·L- 1 vs .(0 .2 9± 0 .19)mmol·L- 1 ,P <0 .0 1;(1.82± 0 .70 )mmol·L- 1 vs.(1.31± 0 .4 8)mmol·L- 1 ,P <0 .0 1]。结论 :脂蛋白胆固醇电泳分析法能直接测定VLDL C ;急性冠脉综合征患者血清VLDL

Objective To evaluate the clinical application of the electrophoresis method for measuring serum cholesterol in lipoproteins and to study the characteristics of lipoprotein fractions in patients with the acute coronary syndrome. Methods Cholesterol in lipoprotein fractions in fasting serum was measured by electrophoresis in 26 patients with the acute coronary syndrome and 20 patients with stable angina pectoris. The results were compared to cholesterol in lipoproteins measured with the regular method. Results There was a good correlation between the methods with coefficients of 0.922, 0.909, and 0.899 for LDL C, HDL C and VLDL C, respectively. The serum levels of TG and VLDL C in the patients with the acute coronary syndrome were significantly higher than those with stable angina pectoris [(0.47±0.33)mmol·L -1 [KG)]vs. (0.29±0.19) mmol·L -1 , P<0.01, (1.82±0.70) mmol·L -1 vs. (1.31±0.48) mmol·L -1 , P<0.01]. . Conclusion The electrophoresis method for quantifying cholesterol in lipoprotein fractions is well correlated with the regular method. The serum levels of VLDL C and TG in patients with the acute conronary syndrome are higher than those with stable angina.