己酮可可碱对人精子获能和顶体反应的影响

Effects of Pentoxifylline on Capacitation and Acrosome Reaction in Human Sperm

目的 探讨不同浓度的己酮可可碱对人精子获能和顶体反应的影响。方法 在体外培养的精子悬液中加入不同浓度的己酮可可碱和孕酮处理 ,通过 CTC染色 ,镜检计数各型精子的百分率。结果 与空白组比较 ,己酮可可碱处理组的获能型精子的百率明显增加 ,终浓度 8mmol·L- 1各处理组顶体反应型的精子百分率不增加 ,而终浓度为 16mmol·L- 1的处理组顶体反应型的精子百分率不明显增加。 结论 己酮可可碱可以促进精子获能 ,在终浓度 8mmol· L- 1 时 PF不诱导精子发生顶体反应 ,但终浓度为 16mmol· L- 1 可促进精子发生顶体反应 (AcrosomeReaction,AR)

OBJECTIVE To investigate the effects of Pentoxiflline (PF) on capacitation and acrosome reaction in human sperm.METHODS Different concentration and progesterone(PF)were added to the liquid containing sperm which were cultured in vitro.Through chlortetracycline(CTC) fluoresence assay,we had counted the percent of different type sperm of each groups under microscope .RESULTS Compared with the blank group,the percent of 'B'type sperm in groups treated with PF was increased greatly,the percent of 'AR'type sperm in groups treated with PF which final concentration on more than 8mmol·L -1 was no increased.But the percent of 'AR'type sperm in the group treated with 16mmol·L -1 PF was increased significantly.CONCLUSION PF can promote the capacitation in human sperm;PF can not induce acrosome reaction when its final concentration is no more than 8mmol·L -1 ,but in the reverse it can stimulate sperm to occur acrosme reaction when its final concentration is 16mmol·L -1 .