丹参注射液对肿瘤浸润淋巴细胞的体外扩增及抗肿瘤作用

Experimental Study of Danshen Injection on TILS' Expansion in Vitro and Cytotoxicity

目的 :观察丹参注射液对肿瘤浸润淋巴细胞(TIL)的体外扩增及抗肿瘤免疫作用 ,为扩大该药的临床应用范围提供依据。方法 :从癌症患者恶性胸水中分离获取TIL和自体瘤细胞 ,观察丹参注射液对TIL体外长期存活、扩增及抗肿瘤作用的影响。结果 :(1)丹参注射液可使TIL体外长期存活 (>17d)。(2)丹参注射液可使TIL在10d内持续扩增并在11～15d保持较高水平。TIL在完全培养剂内则在1周内死亡。(3)扩增后的TIL在体内外的抗肿瘤活性均明显增强 ,作用类似IL 2。结论 :丹参注射液可明显促进TIL的体外扩增及抗肿瘤作用 ,可作为TIL的中药促进剂来替代IL 2。

Objective:Observe the improvement of Danshen Injection (D) on tumor infiltrating lymphocyte (TIL) expansion in vitro and cytotoxicity and provide proof to expand clinical description.Methods:Take TILS and autologous tumor cells separated from the cancer hydrothorax.Observe the promotion of D to induce the expansion of TILS and keep its cytotoxicity.Results:(1)The surviving time of TILS cultured with D prolongs significantly(>17days).(2)D can make TILS continue expanding during 10 days and keep TILS in a comparative large number during 11th ～15th day in culture.TILS in complete medium will die in one week.(3)Expanded TILS can enhance cytotoxicity in vitro and prolong the surviving time of mice with ascites type of HCC.There is no significant difference between D and rIL 2.Conclusion: D can obviously keep TILS living and their expansion in vitro, and can enhance TILS cytotoxicity. D may be used instead of IL 2 in TILS accelerator.