外周血单核细胞中肺炎衣原体特异性抗原的检测

Detection of Chlamydia pneumoniae Specific Antigen in the Peripheral Blood Mononuclear Cell

目的 :肺炎衣原体 (Cpn)特异性抗原检测方法的建立及其应用。方法 :分离人外周血单核细胞 (PBMC) ,用直接免疫荧光 (DIF)检测PBMC中的Cpn特异性抗原 ,同时用间接微量免疫荧光 (MIF)检测Cpn抗体。结果 :颈动脉粥样硬化病人和正常人PBMC中Cpn特异性抗原检出阳性率分别为 6 2 %和 30 % (P <0 .0 1) ;血浆中CpnIgG的检出阳性率分别为 72 %和2 8% ,P值 <0 .0 0 1;血浆中CpnIgM的检出阳性率分别为 16 %和 12 % (P值 >0 .0 5 )。结论 :PBMC中的Cpn特异性抗原和血浆中Cpn -IgG的高检出率均见于颈动脉粥样硬化病人 ,但抗原的检测具有更大的临床意义。

Objective: The aim of the study was to develop the method of detecting Chlamydia pneumoniae (Cpn) specific antigen and its application. Methods: The human peripheral blood mononuclear cells (PBMC) were separated and their specific antigen (Ag) of Cpn were measured by direct immunofluorescence test, at the same time, plasma specific antibodies (IgG?IgM ) to Cpn were measured by microimmunofluorescence test. Results: Cpn-Ag positive rate was 62% (31/50) in carotid atherosclerosis patients and 30% (15/50) in normal control ( P <0.01).Cpn-IgG positive rate was 72% and 28% respectively ( P <0.001). Cpn -IgM positive rate was 16% and 12% respectively ( P >0.05). Conclusion: Cpn-Ag and Cpn -IgG are high positive rate in patients, but detection of Cpn-antigen has more clinical significance.