中药金银花质量标准研究-总黄酮的含量测定

Studies on the Quality Control of Flos Lonicerae-Determination of Total Flavonoids

目的 :建立金银花中总黄酮的含量测定方法。方法 :比色法。结果 :忍冬苷在 0 .0 0 6 0 32～ 0 .0 482 6mg·ml 1线性关系良好 ,平均回收率 99.16 % ,RSD =2 .37%结论 :本方法简便易行 ,重现性好。

OBJECTIVE:To develop a method to determine total flavonoids in Flos Lonicerae. METHOD:Colorimetric method.RESULTS:The calibraion curve of lonicerin had good linear relationship in the range of 0.006032～0.04826mg·ml 1 . The average recovery was 99.16%, and the relative standard deviation was 2.37%. CONCLUSION:The method was simple, easy and reproducible.