抗原诱导豚鼠离体胸主动脉的变态收缩反应(英文)

Antigen-induced Contraction of Guinea Pig Isolated Thoracic Aorta

目的 :研究血管过敏变态反应并观察血管变态反应过程中肥大细胞释放介质的影响。方法 :迅速截取卵蛋白致敏豚鼠的胸主动脉环 (约 3mm)悬挂于 10ml氧饱和的克 亨氏液浴槽中 ,记录不同试剂对抗原诱导的胸主动脉收缩反应影响。结果 :抗原 (卵蛋白 )引起的胸主动脉收缩于 4min达到最大 ,9min约恢复一半 ,至 30min时基本恢复到基线 ,约为最大值的 (8±2 ) % ;苯海拉明对抗原引起的血管收缩反应有延迟作用并显著降低其最大收缩峰值 ,而法莫替丁不影响抗原对血管的收缩作用 ,但可部分减弱苯海拉明的作用 ;5 HT2 受体抑制剂 ,酮色林、环加氧酶抑制剂 ,吲哚美锌和脂氧合酶抑制剂 ,咖啡酸对抗原诱导的血管收缩均有显著的抑制作用 ;当苯海拉明、法莫替丁、酮色林、吲哚美锌和咖啡酸一起孵育胸主动脉环时 ,抗原诱导的血管收缩作用基本被完全抑制。结论 :上述结果表明 ,组胺、5 HT和花生四烯酸代谢物对抗原诱导的胸主动脉血管收缩反应均有调节作用。

OBJECTIVE:To investigate vascular anaphylaxis and examine the role of mast cell associated mediators during vascular anaphylaxis.METHOD:The guinea pigs were actively sensitized with ovalbumin. The thoracic aorta (TA) were isolated and cut into ring segments of about 3mm,and suspended in 10ml organ baths filled with oxygenated Krebs Henselit (K H) solution. We characterized the kinetics of and determined the mediators involved in antigen induced contractions of TA.RESULTS:Ovalbumin (OA) induced contractions of TA, which reached maximum amplitude by 4 min, and delayed to about 50% of maximum by 9 min. By 30 min, the tension was within (8±2)% max. Diphenhydramine (Dip) delayed the onset of contraction and decreased the peak of response.Famotidine (Fam) did not alter antigen induced contractions, but partially reversed effects of Dip. Ketanserin (Ket) or Indomethacin (Ind) or/and Caffeic acid (Caf) significantly inhibited the contraction of TA. Incubated with Ket, Caf, Ind and Dip, the contraction of antigen induced was almost inhibited.CONCLUSIONS:These results suggested that histamine,5 HT,and metabolite of AA mediate antigen induced contraction of TA.