丁香罗勒油气相色谱与气质联用分析

Analysis of Ocimun Oil by GC and CC/MS

目的 :建立丁香罗勒油中丁香酚的含量测定方法和鉴定挥发性化学成分。方法 :气相色谱法 ,4mm× 2m不锈钢柱 ,10 %SE 30为固定液 ,ChromosorbWAW 6 0 80目担体 ,FID检测器 ,柱温 110℃ ,水杨酸甲酯为内标物 ;气质联用 ,以HP 5毛细管柱 ,柱温起始 12 0℃保持 5min后以 5℃ .min-1升温至 15 0℃保持 7min ,检测质荷比范围 10～ 42 5。结果 :丁香酚在 2～ 10mg .mL-1范围内具良好线性关系 ,平均加样回收率为 10 0 .0 8% ,RSD为 0 .95 % ;气质联用鉴定了 39个化合物。结论 :方法简便 ,快速 ,准确 ,可排除其它成分的干扰。

OBJECTIVE: A method was established for the determination of eugenol in the 0cimunc oil by GC and GC/MS method was used to identify the volatile compositions in this article. METHODS: The GC method employed chromosorb WAW with 10% SE 30 as the stationary phase, and the column temperature was 110℃. Hydrogen flame ionization detector was used. GC/MS condition: using programmed temperature gas chromatography (initial temperature was 120℃ maintaining 5min ,and then raising the temperature (5℃.min -1 ) to 150℃ for 7 min),mass to electric charge ratio was 10 to 425. RESULTS: The calibration curve was linear within the range of 2～10mg.mL -1 of eugenol. The results showed that the recoveries and RSD were 100.08% and 0.95%, respectively. The eugenol in 0cimun oil can be determinated accurtely. CONCLUSION: The method is simple,accurate,sensitive and anti interference and GC/MS identified 39 different compositions in ocimun oil.