多粘菌素B对内毒素血症大鼠血流动力学作用

Hemodynamic Effect of Polymyxin B Sulfate on Rats with Endotoxemia

观察多粘菌素B(PMX -B)对内毒素血症大鼠血流动力学的影响 ,并进行了实验。实验分为 :内毒素 (LPS) +多粘菌素B组、LPS +生理盐水组、LPS模型组 ,给大鼠一次性静脉注射内毒素 (0 .5mg kg) ,前 2组分别注入内毒素后 0 .5h静脉给予多粘菌素B(0 .2mg kg)和 1mL生理盐水 (NS) ,LPS模型组大鼠注入内毒素后不作其它处理。给多粘菌素B后 0 5、1、2、4h抽血液 ,检测血浆中LPS、肿瘤坏死因子 (TNF -α)、全血粘度 (WBV)、血球压积 (HRC)的变化。结果表明 :LPS +多粘菌素B组 ,在给内毒素 1h ,全血粘度、红细胞压积明显低于LPS +生理盐水组。

To evaluate the effect of polymyxin B on hemodynamic in endotoxemia rats. Wistar rats were divided into three groups randomly : group 1,the group of LPS( lipopolysaccharide, LPS ) plus polymyxin B were treated with polymyxin B sulfate 1ml(0.2 mg/kg) half an hour after giving endotoxin ;group 2, the group of LPS plus normal saline were treated with normal saline solution 1 ml half an hour after giving endotoxin ; group 3, the group of LPS model were untreated.Wistar rats were first injected intravenously with lipopolysaccharide (LPS, Escherichia coil O111:B4,1mg/kg), Endotoxin, TNF-α, whole blood viscosity,hematocrit of red cells in the blood were detected. After treatment with polymyxin B , whole blood viscosity,hematocrit of red cells in the group of LPS plus polymyxin B were significantly decreased than that of the group of LPS plus normal saline solution. Polymyxin B can reduce level of TNF-α and LPS in circulation and improve hemorheological.