两种内皮细胞分泌血管紧张素II的差异

Difference of Two Types of Vascular Endothelial Cells in Secretion of Angiotensin II

为进一步了解血管内皮细胞 (VEC)在代谢特征上的异质性 ,加深对VEC生物学特性的认识 ,文中应用平行平板流动腔装置 ,将人胚肾小球血管内皮细胞 (HGVEC)和牛肺动脉内皮细胞 (BPAEC)两种不同的EC在同样的剪切流环境中剪切作用 2 4h ,利用放射免疫法检测循环液中血管紧张素II(AngII)分泌量。结果表明 ,HGVEC的AngII平均分泌率 ( 7.5 4± 0 .2 7)pg (cm2 ·h)显著高于BPAEC( 6 .0 8± 0 .11)pg (cm2 ·h) ,且HGVEC的AngII平均分泌率随剪切时间的增加而减少 ,在各时点间的变异相对较大 ;而BPAEC的AngII平均分泌率在各时点的分泌率较为稳定 ,变幅较小。表明这两种内皮细胞在AngII分泌特性上存在异质性。

To understand further the heterogeneity of vascular endothelial cells in metabolism, and enhance knowledge to biological character of vascular endothelial cells, the authors make human glomerulus vascular endothelial cells (HGVECs ) and bovine pulmonary artery endothelial cells (BPAECs) were exposed to steady laminar shear stress for 24 hours by using parallel plate flow chamber apparatus. The secretion production of angiotensin II (Ang II) in circulating medium is detected by radiommunoassay. The results show that Ang II secretion rate averaged for HGVECs(7.54±0.27) pg/(cm 2·h) is significantly higher than that for BPAECs (6.08±0.11) pg/(cm 2·h), and the average Ang II secretion rate for HGVECs decreased with duration of shear stress and its range of variation in different time points is relatively larger; while the average Ang II secretion rate for BPAECs presented has no obvious change with duration of shear stress and its range of variation in different time points was relatively smaller, showing heterogeneity in Ang II secretion character between HGVECs and BPAECs.