番茄子叶的离体再生与移栽

Regeneration and Transplantation of Tomato's Cotyledons

为了尽快地使番茄子叶获得较多的再生植株以及提高再生植株的移栽成活率 ,使番茄的遗传转化研究与应用得以顺利进行 ,以番茄子叶为外植体 ,系统研究了番茄子叶切段大小对诱导出芽的影响 ,生长素IAA和NAA对生根的影响 ,以及移栽土的灭菌处理、空气湿度、再生苗的生根状况等对移栽成活的影响 .结果表明 ,刚展开的子叶横切成 3段时可得到较多的再生芽 ;在MS培养基中添加低浓度的IAA能形成根系发达、生长健壮的再生苗 ;移栽土灭菌处理或再生苗生根差 ,都无法移栽成活 ;空气湿度过高或过低都容易使移栽苗萎蔫 .

In order to make cotyledons of tomato obtain more regenerative plants as soon as possible,raise survival rate of regenerative plants transplanted,and carry out tomato transformation successfully, effects of the size of cotyledon cutting on inducing bud, effects of IAA and/or NAA on rooting and effects of sterilizing soil, air humidity and rooting state on survival of regenerative plants transplanted systematically were studied. The results show that more regenerative buds are obtained by using unfold cotyledons which are cut into three parts. The regenerative plants with well-developed roots and leaves are produced when shoots are cultured in MS in which low concentration IAA is added. Transplanted plants can not survive when soil is sterilized or roots are poor. Higher or lower air humidity is prone to make transplanted plants wither.