应用间接荧光抗体技术快速检测花鲈病原菌——鳗弧菌

An indirect fluorescent antibody technique for detection of Vibrio anguillarum in sea perch (Lateolabrax japonicus)

以花鲈 (Lateolabrax japonicus)弧菌病的病原菌——鳗弧菌 (Vibrio anguillarum) W-1为抗原 ,制备兔抗血清 ;利用异硫氰酸荧光素标记的羊抗兔免疫球蛋白 (FITC-Ig G)为荧光标记二抗 ,并以罗丹明标记的牛血清白蛋白为背景染色 ,建立检测鳗弧菌的间接荧光抗体快速检测技术。应用该技术对人工感染后的花鲈组织 (肌肉、鳃、肠、肾 )样品和养殖水体样品进行了鳗弧菌检测 ,结果显示间接荧光抗体技术不仅可以用于诊断发病的感染花鲈 ,也可用于检测带菌状态或未发病的感染花鲈。

An indirect fluorescent antibody technique (IFAT) incorporating flurescein isothiocyanate (FITC) conjugated anti rabbit globulin goat serum,and rhodamine isothiocyanate conjugated bovine serum albumin (RITC BSA) as background stain has been developed for the detection of Vibrio anguillarum from sea perch ( Lateolabrax japonicus ). Vibro anquillarum of the samples of tissues(muscle,gill,intestine and kidney)in sea perch and cultured water infected artificially were detected by using IFAT.The IFAT,which incorporated a polyclonal antiserum produced in a male New Zealand white rabbit,had a good specificity and sensitivity. The results showed IFAT can be used not only to diagnose clinically diseased fish,but also to recognize subclinically diseased or carrier fish.