叶绿素a光诱导产生O_2~/OH氧自由基

LIGHT-INDUCED FORMATION OF OXYGEN RADICALSO_2~/OH BY CHLOROPHYLL a

将ESR自旋捕俘技术应用于氧的瞬时自由基测定,为叶绿素a Chα光诱导化学行为的研究,提供了更为有力的手段。Harbour和Bolton用自旋捕俘剂DMPO(5,5-Dimethyl-1-pyrroline-N-oxide)首次证明,在光照下叶绿体可产生O_2。他们又用DMPO证明,Chla于光照下有少量OH自由基产生。继而Van

The oxygen radicals O_2/OOH were observed in photochemical reaction of Chlorophyll ain the presence of oxygen. The ESR spectra of DMPO-OOH adduct were recorded directly byusing spin trap technique. The O_2/OOH radicals were formed from oxygen dissolved inhe solutions studied, independent of the aggregation state of Chlorophyll a.