PHB产生菌激光破壁的初步研究

Primary Study on Breaking Up Cell Wall of PHB-Producing Bacteria by Laser Beam

以聚β-羟基丁酸酯(PHB)产生菌为材料,进行激光破碎细胞壁、提取胞内有用物质 PHB 的实验研究。结果表明,以波长为0.3371μm、功率密度为0.43mW/cm^2、频率为1 Hz 的 N_2激光器照射浓度为4.8 mgdry·wtml^(-1)的菌体1分钟,可以达到用化学法同样的破壁效果。

Breaking up cell wall by laser beam and extracting useful material poly-β-hydro-xybutyrate(PHB)from PHB-producing bacterial cells were conducted.It was shown that afterbacterial cells with concentration of 4.8 mg dry wt ml^(-1) were irradiated for one minute by N_2laser beam with wavelength of 0.3371μm,power density of 0.153Mw cm^(2-1) and frequency of 1Hz,cell wall could be disintegrated as well as by chemical treatment.