阴黄证外周血淋巴细胞凋亡及基因调控的研究

A Study of Apoptosis and Gene Regulation in Peripheral Blood Lymphocytes of Yin Jaundice

目的:探讨阴黄证大鼠外用血淋巴细胞凋亡在阴黄发病中的意义。方法:采用α-萘异硫氨酸酯(ANIT)联合大黄制作阴黄证动物模型,运用DNA末端标记法(TUNEL)及流式细胞仪检测明黄证动物模型的凋亡和凋亡率,并用免疫组化法检测Bcl-2、Bcl-X_L、Bax基因表达的变化。结果:阴黄证动物模型有典型的细胞凋亡形态,且凋亡率明显高于正常组、治疗组和阳黄对照组(P<0.01)。阴黄证动物模型的Bcl-2、Bcl-X_L表达减少,而Bax的表达增加。结论:阴黄证存在免疫细胞凋亡,免疫细胞凋亡可能是造成阴黄证免疫功能低下的重要原因之一。

Objective: To study the apoptosis in peripheral blood lymphocytes (PBL) and the pathogenic mechanism of the Yin Jaundice rats. Method: The Yin Jaundice animal model were induced by combing a-nephthylisothiocynate (ANIT) with Deihuang, to demons trate the apoptosis in peripheral blood of rat by TUNEL standing method and deter-min the percentages of apoptosis in PBL of the Yin Jaundice rats by flow cytometer. In addition , the change of expressions in relates Bcl-2, Bcl-XL and Bax gene were observed by using immunohistochemical method. Result: Typical apop-totic cells were observed in the PBL of the Yin Jaundice rats and the percentage of apoptotic cells were significantly higher than those of normal,treated by herb and the Yang Jaundice rats. There was down-regulation in the expression of Bcl-2 and Bcl-XL in PBL of the Yin Jaundice, but the Bax expression remained obviously high. Conclusion: Apoptosis exists in the immunocytes of the Yin Jaundice rats. It is possible the reason that there are low immunity in the Yin Jaundice rats.