我国地带性土壤中产蛋白酶细菌生态分布研究

Study on ecological distribution of protease-producing bacteria in zonal soils

按我国土壤地理地带分布,从北到南采土样32个,从中分得细菌491株,并对每一菌株进行了形态学观察与描述。用透明圈法半定量测定各菌株产蛋白酶的活力。对其中产酶活性较高的菌株进行了摇瓶液体培养,定量测定了产蛋白酶的活力。根据细菌分布的数量与活性,分析了生态条件与产酶细菌分布的规律,得出了一些有意义的结果。

From north China to south China, 32 samples of various types of soil were collected and main ecological factors which might affect the distribution of protease-producing bacteria were studied. Through the study, 491 strains of bacteria were obtained and purified. All of them were systematically observed and their protease-producing activity were half-quantitatively examined. The probable high yielding strains were chosen out and cultured by shaking-flask liquid fermentation method and their protease activity was quantitatively measured. Based on the different protease activity and distribution of bacteria, the relationship between ecological condition and distribution of protease-producing bacteria was studied and following conclusion was obtained: the ecological distribution of protease-producing bacteria has some positive relation with the amounts of soil organic matter and nitrogen.