胃黏膜超微结构、DNA、Zn、Cu和LPO研究慢性胃炎分类

Detection of ultrastructural changes and contents DNA, Zn, Cu, and LPO in subgroups of chronic gastritis

目的:探索慢性胃炎中无症CSG、无症CAG、有症F型、有症CSG和有症CAG的病理生理学渊由。 方法:对188例患者,应用透射电镜、能谱仪、图像分析仪与化学发光法等现代科学技术手段,同步检测胃黏膜上皮细胞超微结构、微量元素、DNA和SOD,以及LPO。 结果:胃黏膜上皮细胞核Zn、Cu、DNA和血清LPO含量,健康对照组(7.6±0.4,58.4±0.3,12.6±2.7,2.6±0.6),无症CSG组(7.8±0.3,58.6:0.4,13.0±3.1,2.9±0.4),无症CAG组(7.8±0.3,58.7±0.3,14.3±2.8,3.1±0.4),有症F型组(7.9±0.4,58.7±0.5,13.5±4.6,2.9±0.7),有症CSG组(8.1±0.5,58.9±0.5,15.2±3.2,4.2±0.7),有症CAG组(8.6±0.4,59.3±0.5,16.5±3.1,4.5±0.6);而线粒体Zn、Cu和胃黏膜SOD含量,健康对照组(9.2±0.5,58.3±0.3,170.5±6.1),无症CSG组(8.9±0.5,58.2±0.3,167.2±5.3),无症CAG组(8.8±0.4,57.5±0.2,166.1±4.2),有症F型组(8.9±0.5,58.0±0.3,167.9±5.7),有症CSG组(8.6±0.5,57.8±0.3,163.3±5.6),有症CAG组(8.3±0.4,57.5±0.3,161.2±4.3),组间均有显著性差异,P<0.05～0.001。胃黏膜上皮细胞超微结构与此发生同步变化。 结论:有病有症、有病无症、无病有症都发生在胃黏膜上皮细胞超微结构及其相关的生物活性物质的质量变化的基础上。

AIM: To explore the pathophysiologic mechanisms for chronic superficial gastritis (CSG) without symptoms, chronic atrophic gastritis (CAG) without symptoms, F type with symptoms, CSG with symptoms and CAG with symptoms.METHODS: Ultrastructural changes, trace elements, DNA content, SOD and LPO were detected- synchronously in gastric mucosa of 188 patients.RESULTS: The contents of Zn, Cu, DNA and LPO in each group were showed as below: Health control group(7.6±0.4,58.4±0.3,12.6±2.7,2.6±0.6), CSG without symptoms group(7.8± 0. 3,58.6 ± 0.4,13.0 ± 3. 1,2.9 ± 0.4) , CAG without symptoms group(7.8±0.3,58.7 ± 0.3,14.3±2.8,3.1 ±0.4), F type with symptoms group(7 9 ± 0.4,58.7 ± 0.5, 13.5 ±4.6,2.9 ±0.7), CSG with symptoms group(8.1 ±0.5, 58.9±0.5,15.2±3.2,4.2±0.7), and CAG with symptoms group(8.6±0.4,59.3 ±0.5,16.5±3.1,4.5 ±0.6); While the content of Zn, Cu in mitochonondria and SOD in gastric mucosa of each group were showed as below: Health control group(9. 2 ± 0.5,58.3 ± 0. 3, 170.5 ± 6. 1), CSG without symptoms group (8.9±0.5,58.2±0.3,167.2±5.3), CAG without symptoms group (8.8 ±0.4,57.5±0.2,166.1± 4.2) ;F type with symptoms group (8.9±0.5,58.0±0.3,167. 9±5.7), CSG with symptoms group (8.6 ±0.5,57.8± 0.3, 163.3 ±5.6); CAG with symptoms group (8.3 ± 0.4,57.5 ±0.3,161.2 ± 4.3). There were significant differences among the above groups, ( P < 0. 05 - 0. 001 ) with synchronous ultrastructureal changes in gastric mucosa.CONCLUSION:Disease with or without symptoms, 'normal' with symptoms occur on the basis of the quantitative changes of gastric mucosa and related bioactive substances.