慢性胃炎与胃癌胃黏膜超微结构及其分子生物学的比较研究

Ultrastructural and molecular biological changes of chronic gastritis and gastric cancer: a comparative study

目的:慢性胃炎与胃癌胃黏膜超微结构及其分子生物学的比较研究.方法:对168例慢性浅表性胃炎、慢性萎缩性胃炎和胃癌患者胃黏膜,采用组织化学染色、扫描电镜附能谱仪、透射电镜附能谱仪、图像分析仪、放射免疫法和化学发光法等现代科学技术手段,同步检测胃黏膜和胃黏膜上皮细胞超微结构、微量元素、DNA、cAMP和SOD,以及3H-TdR LCT、LPO.结果:上皮细胞核质比＞1的发生率、核分叶的发生率、染色质间颗粒密集的发生率、核仁肥大的发生率、细胞核DNA、Zn、Cu和血清LPO,健康对照组组为0.0,0.0,6.7,0.0,12.6±2.7,7.6±0.4,58.4±0.3,2.6±0.6,CSG组为5.7,2.9,7.4,2.9,15.2±3.1,8.1±0.5,58.9±0.5,4.2±0.7,CAG组为31.3,29.7,45.3,42.2,16.5±3.1,8.6±0.4,59.3±0.5,4.5±0.6,CA组为100.0,100.0,72.2,50.0,30.7±8.2,8.8±0.3,59.5±0.4,6.8±1.6;而线粒体Zn、Cu,胃黏膜cAMP和SOD、3H-TdR LCT,健康对照组组为9.2±0.5,58.3±0.3,15.9±1.5,170.5±6.1,1079.7±227.4,CSG组为8.6±0.5,57.8±0.3,14.6±1.8,163.3±5.6,867.3±240.5,CAG组为8.3±0.4,57.5±0.3,13.4±1.8,161.2±4.3,800.9±221.8,CA组为8.9±0.4,57.1±0.3,10.2±3.9,152.2±3.8,325.7±186.8,组间有显著性差异,P＜0.05～0.01.结论:慢性胃炎与胃癌间在胃黏膜超微结构及其分子生物学上还是有显著性差异的;慢性萎缩性胃炎必须在机体内环境改变的基础上,结合外环境不良因素的刺激,才有可能发展成胃癌.

AIM: To carry out a comparative study on ultrastructural and molecular biologic changes of chronic gastritis (CG) and gastric cancer(GC).METHODS: By use of histochemical staining, SEM with EDAX, TCM with EDAX, image analysis technique, RIA and chemiluminescence method, gastric mucosa of 168 patients were synchronously analyzed in morphology, trace elements, DNA, cAMP, SOD 3H-TdR LCT and serum LPO.RESULTS:The incidences in epithelial nucleoplasmic ratio > 1, lobulated nuclei, chromatin dense, nucleolar hypertrophy, and the content of DNA, Zn, Cu in nuclei and serum LPO of each group were showed as below: healthy control group (0.0,0.0,6.7,0.0,12.6±2.7,7.6±0.4,58.4± 0.3,2.6±0.6),CSG group (5.7,2.9,7.4,2.9,15.2 ± 3.1,8.1 ±0.5,58.9±0.5,4.2±0.7),CAG group (31.3,29.7,45.3,42. 2,16.5±3.1,8.6±0.4,59.3±0.5,4.5±0.6),CA group (100. 0,100.0,72.2,50.0,30.7 ±8.2,8. 8 ±0.3,59.5 ±0.4,6. 8 ± 1. 6); whereas the contents of Zn, Cu in mitochondria and cAMP , SOD in gastric mucosa, and 3H-TdR LCT of each group were showed as below: healthy control group (9.2 ± 0.5,58.3 ±0.3,15.9 ± 1.5,170.5 ± 6.1,1079.7 ± 227.4), CSG group (8.6± 0.5,57.8 ±0.3,14.6 ± 1.8,163.3 ± 5.6,867.3 ± 240.5),CAG group (8.3±0.4,57.5±0.3,13.4± 1.8,161.2± 4.3,800.9±221.8),CA group (8.9±0.4,57.1 ±0.3,10.2 ±3. 9,152. 2 ±3. 8, 325. 7 ± 186. 8). There were significant differences among the groups ( P < 0.05 - 0.01).CONCLUSION: There is a significant difference between CGand GC in their ultrastructure and molecular biology. Only in proper condition of the changes of internal environment of organism in combination with the induction of harmful factors of external environment is chronic atrophic gastritis likely to develop into gastric cancer.