U73122和三氟吡啦嗪对柴胡皂甙(I)促胰腺腺泡酶分泌的影响

EFFECTS OF U73122 AND TRIFLUOPERAZINE ON AMYLASE SECRETION STIMULATED BY SAIKOSAPONIN(I) IN RAT PANCREATIC ACINI

应用碘淀粉比色法检测淀粉酶活性技术 ,以卡巴可 ( CCh)及八肽胆囊收缩素 ( CCk-8)为阳性对照药 ,研究了磷脂酶 C( PLC)抑制剂 U731 2 2和钙调素 ( Ca M)抑制剂三氟吡啦嗪 ( trifluoperazine)影响柴胡皂甙 ( I) [SA( I) ]刺激大鼠胰腺腺泡酶分泌的剂量依赖性和分泌动力学特征 .U731 2 2抑制 SA( I)和 CCh促酶分泌有相似的剂量依赖性 ,1 0 - 5mol·L- 1 U731 2 2分别抑制了相同浓度 SA( I)和 CCh作用的 5 7.4 %和 6 1 .5 % .U731 2 2抑制 SA( I)促酶分泌动力学较对 CCh的抑制缓慢且持续时间较长 .trifluoperazine抑制 SA( I)促酶分泌与对 CCk-8的抑制有相似的剂量依赖性 ,高浓度对 SA( I)作用的抑制更显著 .结果表明 ,SA( I)活化 PLC和 Ca

Using the measurment of amylase activity, influences of U73122, an inhibitor of phospholipase C(PLC), and trifluoperazine, an inhibitor of calmodulin (CaM), on amylase secretion stimulated by carbachol(CCh), cholecystokinin octapeptide (CCk-8) and saikosaponin(I) in rat pancreatic acini were studied. The inhibitions of U73122 on amylase secretion stimulated by SA(I) and CCh had the similar dose-dependent characteristics, 10 -5mol·L -1U73122 inhibited the actions of SA(I) and CCh by 57.4% and 61.5% respectively. The inhibitory kinetics of U73122 on amylase secretion stimulated by SA(I) was slower and sustained. The inhibitions of trifluoperazine on amylase secretion stimulated by SA(I) and CCk-8 had the similar dose-dependent characteristics, high concentration trifluoperazine inhibited strongly the action of SA(I). These results indicate that activating PLC and CaM are involved in signaling transduction pathway of amylase secretion stimulated by SA(I).