异丙酚对不同麻醉时期大鼠脑NO及NOS的影响

Effects of Propofol on Nitric Oxide and Nitric Oxide Synthase of Rat Brain at Different Anesthesia Phases

目的 观察异丙酚对不同麻醉时期 SD大鼠皮质、海马、脑干、小脑一氧化氮合酶 (NOS)活性和一氧化氮 (NO)产量的动态影响 ,探讨异丙酚麻醉作用的机制。 方法 4 0只 SD大鼠随机分成 5组 :对照组、诱导期组、麻醉期组、恢复期组和清醒期组。对照组腹腔注射生理盐水 10 m l/kg,其余各组注射异丙酚 10 0 m g/kg,在不同麻醉时期断头取脑 ,用分光光度法测定各组大鼠皮质、海马、脑干、小脑 NOS活性和 NO产量。 结果 (1)与对照组比较 ,诱导期组与麻醉期组皮质、海马、脑干、小脑 NOS活性均有不同程度的降低 (P<0 .0 1) ;与麻醉期组比较 ,恢复期组各脑区 NOS活性明显回升 (P<0 .0 1或 P<0 .0 5 ) ,清醒期组 NOS活性继续回升 ;与对照组比较 ,皮质、脑干NOS活性无明显差异 (P>0 .0 5 ) ;(2 )与对照组比较 ,诱导期组皮质、海马、脑干、小脑 NO产量均降低 ,其中脑干、小脑下降明显 (P<0 .0 5 ) ,麻醉期组进一步降低 (P<0 .0 1) ;恢复期组各脑区 NO产量回升 ,其中脑干 NO产量上升明显 (P<0 .0 5 ) ;清醒期组各脑区 NO产量显著上升 (P<0 .0 1) ;与对照组比较 ,皮质、海马、脑干 NO产量无明显差异 (P>0 .0 5 )。 结论 大鼠腹腔注射异丙酚 10 0 mg/kg可降低不同麻醉时期大鼠各脑区 NOS活性和 NO产量 ,此与行为学?

Objective\ To investigate effects of propofol on the dynamic changes of nitric oxide(NO) and nitric oxide synthase(NOS) activity at each anesthesia phase in rat brain.\ Methods\ Forty rats were divided randomly into five groups.\ The animals were injected intraperitoneally(IP) propofol 100 mg/kg or normal saline 10 ml/kg(control group) respectively.\ The rats were immediately decapitated \{before\}(induction group) or after righting reflex disappearance(anesthesia group), when righting reflex \{appeared\} again(recovery group), or one hour after(awaken group).\ NO production and NOS activity were estimated by spectrophotometer.\ Results\ After IP propofol 100 mg/kg, righting reflex disappeared and NOS activity and NO production of cortex, hippocampal, brain stem, cerebellum were inhibited significantly(P<0 01).\ Following righting reflex appeared again, NOS activity and NO production rehabilitated again.\ Conclusion\ The changes of NO production and NOS activity are in accord with the behavior change of rats.\ NO production in rat brain may be as secondly messager on the central inhibitory effects of propofol.\;