胰岛素样生长因子-1受体在实验性肝纤维化中的表达及IL-10的干预作用

Expression of Insulin-like Growth Factor 1 Receptor and its Intervention by IL-10 in Experimental Liver Fibrosis

目的 观察胰岛素样生长因子 - 1受体 (IGF- 1R)在实验性大鼠肝纤维化过程中的表达情况及白细胞介素 - 10 (IL - 10 )对肝纤维化大鼠 IGF- 1R表达的影响。 方法 建立 CCl4 诱导大鼠肝纤维化模型并行 IL - 10干预实验。取对照组、模型组及 IL - 10干预组大鼠肝脏组织 ,采用 SP免疫组织化学方法检测分析在肝纤维化进程不同阶段大鼠肝组织 IGF- 1R的表达情况。 结果 经 CCl4 诱导成功建立大鼠肝纤维化模型。随着肝纤维化程度的加重 ,IGF- 1R在肝组织中阳性表达明显增强 ;经 Ridit分析 ,对照组与模型组 IGF- 1R阳性表达差异有显著性 (P<0 .0 1) ;IL - 10干预组 IGF- 1R阳性表达较模型组明显减弱 ,差异有显著性 (P<0 .0 5 ) ,且随 IL - 10干预时间的延长 ,IGF- 1R在肝组织中阳性表达逐渐减弱。 结论 随着肝纤维化程度的进展 IGF- 1R阳性表达增强 ,外源性 IL - 10可降低 CCl4 诱导大鼠肝纤维化中 IGF- 1R的表达。

Objective\ To clarify the expression of insulin\|like growth factor 1 receptor(IGF\|1R) and its intervention by interleukin\|10(IL\|10) in the course of experimental rat liver fibrosis.\ Methods\ Experimental rat liver fibrosis model was established and the liver tissues were obtained from model group, IL\|10 treated group and control group in different stages respectively.\ The expression of IGF\|1R at different stages in the development of liver fibrosis was measured by SP immunohistochemistry on each group.\ Results\ The CCl\-4\|induced experimental rat liver fibrosis model was successfully established.\ The positive expression of IGF\|1R was increased with the development of fibrosis, Ridit analysis indicated that there was singnificant difference between control and model groups(P<0 01).\ However, the positive expression of IGF\|1R in IL\|10 treated group was decreased when compared with control group.\ \{Conclusion\}\ The expression of IGF\|1R increases in the course of liver fibrosis; IL\|10 can decrease the \{expression\} of IGF\|1R in experimental rat fibrosis.\;