CD_(154)反义RNA诱导Jurkat细胞的凋亡

Apoptotic Effect of CD_(154) Antisense RNA on Jurkat Cell

目的 探讨 CD1 54反义 RNA诱导 Jurkat细胞凋亡的作用。 方法 应用 RT- PCR扩增人 CD1 54膜外段基因 ,以及 T- A克隆技术和亚克隆技术构建 CD1 54反义 RNA的真核表达载体 ,并将其转染入 Jurkat细胞中。应用电镜及流式细胞仪 (FCM)检测观察 Jurkat细胞的凋亡指标。 结果 电镜观察 CD1 54反义 RNA转染的 Jurkat细胞内可见凋亡小体 ,并且 FCM检测显示一明显的凋亡峰。 结论 CD1 54反义 RNA可诱导 Jurkat细胞凋亡。

Objective\ To investigate the effects of CD\-\{154\} antisense RNA on Jurkat cell.\ \{Methods\} CD\-\{154\} gene extracelluar domain sequence was amplified by RT\|PCR.\ Then, the PCR product was cloned and subcloned with T\|A cloning and subcloning techniques.\ After the vector was transfected into Jurkat cells, apoptotic data were obtained with flow cytometry(FCM) and electron microscope techniques.\ \{Results\}\ Apoptotic bodies were found in the transfeced Jurkat cells under electron microscope; and FCM analysis showed a significant apoptotic peak.\ Conclusion\ CD\-\{154\} antisense RNA can induce apoptosis of Jurkat cells.\;